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KMID : 0380620200520060622
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Korean Journal of Food Science and Technology
2020 Volume.52 No. 6 p.622 ~ p.626
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Canavalia gladiata regulates the immune responses of macrophages differently depending on the extraction method
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Lee Ha-Nul
Kim Young-Min
Jang Ah-Ra
Kim Young-Ran
Park Jong-Hwan
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Abstract
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Recent studies have suggested that Canavalia gladiate, a dietary food and traditional folk medicine, has promising pharmaceutical potential, but the effects have mostly been demonstrated using its organo-soluble extract. To date, its immunomodulatory effect depending on the extraction method is unclear. Here, the immune responses of macrophages to C. gladiate and the underlying mechanisms were studied. C. gladiate hot water extract (CGW) induced cytokine production in bone marrow-derived macrophages (BMDMs) in a dose-dependent manner, whereas its ethanolic extract (CGE) did not. Immunoblotting analysis also showed that CGW activated nuclear factor (NF)-κB and mitogen-activated protein kinases (MAPKs). Moreover, an inhibitor assay revealed the involvement of NF-κB, p38, and JNK, but not ERK, in CGW-induced cytokine production. CGE inhibited lipopolysaccharide-stimulated production of pro-inflammatory cytokines and activation of NF-κB and MAPKs in BMDMs. The results suggest that C. gladiate regulates the immune responses of macrophages differently depending on the extraction method.
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KEYWORD
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Canavalia gladiate, cytokine, nuclear factor-κB, mitogen-activated protein kinase, macrophage
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